ARHGDIB antibody (N-Term)

Catalog No. ABIN499142

Product Details anti-ARHGDIB Antibody

Target: ARHGDIB (rho GDP Dissociation Inhibitor (GDI) beta (ARHGDIB))

Binding Specificity: N-Term

Reactivity: Human, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGDIB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: TEKAPEPHVE -C

Specificity: Recognizes ARHGDIB.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Pig, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_001166.3. Genename: ARHGDIB

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 26 kDa in lysates of cell lineJurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARHGDIB

Target: ARHGDIB (rho GDP Dissociation Inhibitor (GDI) beta (ARHGDIB))

Alternative Name: ARHGDIB / D4-GDI (ARHGDIB Products)

Synonyms: ARHGDIB antibody, zgc:65966 antibody, arhgdib antibody, MGC69067 antibody, gdia2 antibody, rhodgi-2 antibody, rhodgi2 antibody, D4 antibody, GDIA2 antibody, GDID4 antibody, LYGDI antibody, Ly-GDI antibody, RAP1GN1 antibody, RhoGDI2 antibody, Gdid4 antibody, Rho GDP dissociation inhibitor (GDI) gamma antibody, Rho GDP dissociation inhibitor (GDI) beta S homeolog antibody, Rho GDP dissociation inhibitor beta antibody, Rho GDP dissociation inhibitor (GDI) beta antibody, Rho, GDP dissociation inhibitor (GDI) beta antibody, arhgdig antibody, arhgdib.S antibody, ARHGDIB antibody, arhgdib antibody, Arhgdib antibody

Background: D4-GDI belongs to the family of Rho GDIs or GDP dissociation inhibitors for the Rho/Rac family of small G proteins, consisting of RhoGDI 1, D4 GDI/RhoGDI2 and Rho GDI3. It inhibits the release of GDP from the Rho proteins, essential for GTP loading and activation of GTPase activity. D4 GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas mediated apoptosis.Synonyms: GDIA2, GDID4, Ly-GDI, RAP1GN1, Rho GDI 2, Rho GDP-dissociation inhibitor 2, Rho-GDI beta, RhoGDI2

Molecular Weight: 23.0kDa (NP_001166.3)

Gene ID: 397

NCBI Accession: NP_001166

UniProt: P52566

Pathways: Caspase Cascade in Apoptosis